The east end of the line (MP 38.7) is adjacent to the Lewes Beach State Park. heading west-southwest, the line bridges over the Lewes-Rehoboth Canal to Lewes (MP 38.0), where there is a siding on the south side of the line (with a boarding platform) and a west-facing spur on the north side, continuing west-southwest past Nassau (MP 35.6), where there is a siding on the north side, followed by a west-facing spur that once served a milk company, the former location of the depot on the north side, and a grade crossing.
At Cool Spring (MP 31.8), there is a west-facing spur on the south side, where the depot used to be, and the line makes a grade crossing with SR 9. At Harbeson (MP 29.6), there is a grade crossing, the former location of the depot on the north side of the line, and a siding on the north side of the line, with the Allen's Chicken plant just to its north. The interchange with the Conrail Indian River Secondary Track, at Georgetown, is another five miles further west.